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KMID : 0363620080290040171
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 4 p.171 ~ p.179
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Screening of the Bufonis Venenum on Hep G2 Cells
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Kang A-Mi
Lim Seong-Woo
Kim Bo-Ram
Kim Sung-Uk
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Abstract
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Objective: Bufonis Venenum is the traditional Korean medicine Chan Su, which is obtained from the skin and parotid venom gland of the toads. It has been used for myocardial diseases, inflammation diseases, pain relief, cancer and others. The main components of BV are cinobufotoxin, cinobufalin, bufalin and others. Of these, bufalin, the major active ingredient of BV, has been reported to induce apoptosis and to possess anti-tumor effects. There was no report of anti-tumor screening of BV on hepatic cancer and which signaling pathway can be involved. In order to examine the effect of BV on hepatic cancer and the related signaling pathway with BV-induced apoptosis, human Hep G2 cells were used.
Methods: Analysis of apoptosis was confirmed by MTT assay. BV decreased cell viability in a dose and duration dependent manner. To observe which signaling molecules will be activated by BV, phosphorylation of MAPK (p38, ERK, JNK), caspase 8 and caspase 9 were examined by Western blot analysis.
Results: The phosphorylation levels of p38 started to increase at 5 min after addition of 5 /ml of BV and sustained to increase until 48 hours. The phosphorylation levels of other MAPK (ERK and JNK), caspase 8 and caspase 9 increased in a time-dependent manner. These imply that BV may activate different signaling pathways, MAPK, caspase 8 and caspase 9. These results propose that BV may induce apoptosis on Hep G2 cells through the activation of MAPK, caspase 8 and caspase 9.
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KEYWORD
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Bufonis Venenum, Hep G2 cell, apoptosis, MAPK, caspase 8, caspase 9
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